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Molecular cloning and sequence analysis of cDNA coding for canine gastrin
S C Kang1, K L Agarwal, O J Yoo
1Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Seoul.
Biochemistry International
|March 1, 1989
Summary
Researchers isolated and sequenced canine preprogastrin cDNA, finding 83% homology with other species. This study details the canine preprogastrin structure, aiding comparative studies of this important hormone.
Area of Science:
- Gastroenterology
- Molecular Biology
- Comparative Genomics
Background:
- Gastrin is a key hormone regulating gastric acid secretion.
- Understanding gastrin's structure across species provides insights into its function and evolution.
Purpose of the Study:
- To isolate and sequence canine preprogastrin cDNA.
- To compare the canine preprogastrin sequence with homologous sequences from other species.
Main Methods:
- Isolation and sequencing of cDNA clones from a canine antral cDNA library.
- Bioinformatic analysis and sequence comparison.
Main Results:
- Canine preprogastrin cDNA was successfully isolated and sequenced.
- The sequence showed 83% homology in the coding region and conserved noncoding regions compared to porcine, human, and rat gastrin.
- Canine preprogastrin consists of 104 amino acids, including a signal peptide, prosegment, gastrin sequence, glycine (amide donor), and arginine residues.
Conclusions:
- Canine preprogastrin shares significant sequence homology with other mammalian species.
- The identified structure provides a basis for further functional and evolutionary studies of gastrin.
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