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Establishment and application of a flow cytometry-based method for detecting histone acetylation levels
Jian-hui Li1, Chun-fu Wang1, Fan Bai1
1Department of Infectious Diseases, Tang-du Hospital affiliated to Fourth Military Medical University, Xi'an 710038, China.
Yi Chuan = Hereditas
|September 23, 2016
Summary
Researchers developed a reliable flow cytometry method to detect histone acetylation levels. This technique is fast, convenient, and applicable to both basic and clinical research for anticancer drug development.
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Background:
- Histone deacetylase inhibitors (HDACi) are emerging anticancer drugs.
- A reliable method for detecting histone acetylation is lacking for basic and clinical research.
Purpose of the Study:
- To develop and optimize a flow cytometry-based method for detecting histone acetylation levels.
- To validate the method's reliability, speed, and convenience in various research settings.
Main Methods:
- Optimized sample processing temperature, permeabilization, antibody dose, and incubation time.
- Utilized whole blood and peripheral blood mononuclear cells.
- Applied the method in in vitro experiments and a clinical trial of Chidamide (HDACi).
Main Results:
- Established an optimized flow cytometry protocol for histone acetylation detection.
- Demonstrated the method's reliability and efficiency using whole blood and PBMCs.
- Validated the method's applicability in preclinical and clinical studies of an HDAC inhibitor.
Conclusions:
- The developed flow cytometry method provides a reliable, fast, and convenient approach for quantifying histone acetylation.
- This method is suitable for both basic research and clinical applications, particularly in studies involving HDAC inhibitors.
