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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

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Noncoding RNA Interplay with the Genome.

Davide Gabellini¹

¹Dulbecco Telethon Institute and Division of Regenerative Medicine, San Raffaele Scientific Institute, Milano, Italy. gabellini.davide@hsr.it.

Methods in Molecular Biology (Clifton, N.J.)

|September 24, 2016

Summary

This summary is machine-generated.

Most of our genome produces noncoding RNA, including numerous long noncoding RNAs (lncRNAs). These molecules regulate gene expression, and new methods aid their discovery and characterization.

Keywords:

Bioinformatics ChIRP Chart LncRNA RAP RIP RNA pull-down RNA-seq

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Chromatin Isolation by RNA Purification ChIRP

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Published on: March 25, 2012

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

The majority of the human genome is transcribed into RNA molecules.
Most of these RNA molecules are noncoding.
Long noncoding RNAs (lncRNAs) represent the most abundant and functionally diverse class of noncoding RNAs.

Purpose of the Study:

To describe bioinformatics approaches crucial for identifying long noncoding RNAs (lncRNAs).
To outline molecular techniques for characterizing the structure and function of lncRNAs.
To highlight the significance of lncRNAs as gene expression regulators.

Main Methods:

Bioinformatic analysis for lncRNA identification and discovery.
Molecular biology techniques for lncRNA structure determination.
Functional assays to elucidate lncRNA roles in gene regulation.

Main Results:

Established bioinformatics pipelines for robust lncRNA discovery.
Developed and validated molecular methods for lncRNA structure-function studies.
Demonstrated the critical role of lncRNAs in regulating gene expression at multiple levels.

Conclusions:

lncRNAs are a major class of regulatory molecules with diverse functions.
Advancements in bioinformatics and molecular techniques are essential for lncRNA research.
Further investigation into lncRNAs promises significant insights into gene regulation and cellular processes.