A fully integrated electrochemical biosensor platform fabrication process for cytokines detection.

Area of Science:

• Biomedical Engineering
• Biosensor Technology
• Analytical Chemistry

Background:

• Heart failure (HF) patients undergoing left ventricle assisted device (LVAD) implantation experience acute inflammation.
• Interleukin-1b (IL-1b) and interleukin-10 (IL-10) are key biomarkers secreted during this inflammatory response.
• Early detection of these cytokines is crucial for predicting post-operative complications.

Purpose of the Study:

• To develop an integrated electrochemical biosensor platform for sensitive, simultaneous detection of IL-1b and IL-10.
• To enable detection of these cytokines at minute concentrations for early inflammation prediction.
• To reduce analysis time for critical biomarker monitoring in LVAD patients.

Main Methods:

• Fabrication of a silicon-based biosensor with eight gold working microelectrodes (WEs).
• Functionalization of gold WEs using monoclonal antibodies (mAbs) against IL-1b and IL-10 via 4-carboxymethyl aryl diazonium (CMA).
• Characterization using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).

Main Results:

• The biosensor demonstrated high sensitivity for detecting IL-1b and IL-10 within the 1-15 pg/mL range.
• Simultaneous detection of both cytokines was achieved with no observed interference from other cytokines.
• The platform confirmed successful electroaddressing and characterization of modified gold WEs.

Conclusions:

• The developed electrochemical biosensor platform offers a promising tool for multiplexed cytokine detection.
• It significantly decreases analysis time, providing rapid data for clinical decision-making.
• This technology can aid in the early prediction of inflammation following LVAD implantation.