Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Folding a broken genome: the versatile roles of cohesin in genome maintenance.

Nature reviews. Genetics·2026
Same author

Cellular replisomes are powered by flex-fuel motors for unwinding DNA.

Nature communications·2026
Same author

Cohesin activity accelerates the homology search.

bioRxiv : the preprint server for biology·2026
Same author

A modular framework for automated segmentation and analysis of AFM imaging of chromatin organization.

Nucleic acids research·2026
Same author

Searching for sequence features that control DNA cyclizability.

PNAS nexus·2026
Same author

Intelligent fluorophores: navigating biological complexity through adaptive single-molecule imaging.

Science bulletin·2026

Related Experiment Video

Updated: Mar 14, 2026

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

9.9K

Single-molecule fluorescence microscopy of native macromolecular complexes.

Vasudha Aggarwal1, Taekjip Ha2

  • 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, USA.

Current Opinion in Structural Biology
|September 24, 2016
PubMed
Summary

Single-molecule pull-down assays offer precise insights into the dynamic nature of biological macromolecular complexes. This review highlights advancements in these techniques for studying native protein, lipid, and nucleic acid assemblies.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

13.3K

Related Experiment Videos

Last Updated: Mar 14, 2026

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

9.9K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

13.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysics

Background:

  • Macromolecular complexes are fundamental to biological processes.
  • Their dynamic and heterogeneous nature necessitates advanced study methods.
  • Single-molecule techniques provide accurate measurements of complex properties.

Purpose of the Study:

  • To review recent advancements in single-molecule pull-down (SMPD) methods.
  • To discuss biological systems investigated using SMPD.
  • To highlight the utility of SMPD for probing native macromolecular complexes.

Main Methods:

  • Combining co-immunoprecipitation principles with single-molecule fluorescence microscopy.
  • Development and application of novel single-molecule pull-down assays.
  • In situ and semi in vivo analyses of macromolecular assemblies.

Main Results:

  • SMPD methods enable detailed characterization of complex composition and stoichiometry.
  • Dynamic changes in complex conformations can be accurately measured.
  • Various biological systems, including protein-lipid and protein-nucleic acid complexes, have been successfully studied.

Conclusions:

  • Single-molecule pull-down assays are powerful tools for dissecting complex biological machinery.
  • These methods provide unprecedented resolution for studying native macromolecular complexes.
  • Continued development of SMPD will advance our understanding of cellular processes.