Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Radioimmunoassay for benzo[a]pyrene.

N Y Kado, E T Wei

    Journal of the National Cancer Institute
    |July 1, 1978
    PubMed
    Summary

    A novel radioimmunoassay (RIA) for benzo[a]pyrene (BP) was developed. This assay can detect trace amounts of BP and shows high specificity, offering potential for quantifying carcinogen exposure in humans.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    TRPM8 agonist (cryosim-1) gel for scalp itch: a randomised, vehicle-controlled clinical trial.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2022
    Same author

    Isolation and identification of a direct-acting mutagen in diesel-exhaust particulates.

    Environmental science & technology·2012
    Same author

    Indoor-Outdoor Concentrations and Correlations of PM10-Associated Mutagenic Activity in Nonsmokers' and Asthmatics' Homes.

    Environmental science & technology·2011
    Same author

    How best to fight that nasty itch - from new insights into the neuroimmunological, neuroendocrine, and neurophysiological bases of pruritus to novel therapeutic approaches.

    Experimental dermatology·2005
    Same author

    Adverse health effects of selenium in humans.

    Reviews on environmental health·2002
    Same author

    Cutaneous expression of corticotropin-releasing hormone (CRH), urocortin, and CRH receptors.

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2001

    Area of Science:

    • Environmental Chemistry
    • Toxicology
    • Immunochemistry

    Background:

    • Benzo[a]pyrene (BP) is a carcinogenic polycyclic aromatic hydrocarbon.
    • Accurate quantification of BP exposure is crucial for public health.
    • Existing methods may lack sensitivity or specificity for certain applications.

    Purpose of the Study:

    • To develop and validate a sensitive and specific radioimmunoassay (RIA) for benzo[a]pyrene (BP).
    • To assess the specificity of the developed RIA against related polycyclic aromatic hydrocarbons.
    • To evaluate the potential utility of the RIA for quantifying BP in biological samples.

    Main Methods:

    • Synthesis of a benzo[a]pyrene-bovine serum albumin conjugate for immunization.
    • Generation of specific antisera in New Zealand White rabbits.
    • Development of a radioimmunoassay using radiolabeled and unlabeled BP for standard curve construction and competitive binding assays.

    Main Results:

    • The developed RIA demonstrated high sensitivity, reliably detecting as little as 0.4 pmoles (0.1 ng) of BP.
    • The antiserum exhibited high specificity for BP, with 3-hydroxybenzo[a]pyrene showing only 20% relative cross-reactivity.
    • The RIA successfully quantified BP in pooled human serum at concentrations as low as 1 ng/ml.

    Conclusions:

    • A sensitive and specific radioimmunoassay for benzo[a]pyrene has been successfully developed.
    • The RIA shows potential for accurate quantification of absorbed benzo[a]pyrene doses in human samples.
    • This assay could be a valuable tool in environmental monitoring and toxicological studies.

    Related Experiment Videos