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A New Portable In Vitro Exposure Cassette for Aerosol Sampling

A New Portable In Vitro Exposure Cassette for Aerosol Sampling

Published on: February 22, 2019

A new fluorescence-based method for characterizing in vitro aerosol exposure systems.

Sandro Steiner¹, Shoaib Majeed¹, Gilles Kratzer¹

¹Philip Morris International R&D, Philip Morris Products S.A. (part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000, Neuchatel, Switzerland.

Toxicology in Vitro : an International Journal Published in Association with BIBRA

|September 25, 2016

Summary

A new fluorometry method accurately measures liquid aerosol delivery in in vitro systems. This robust technique aids in characterizing aerosol exposure systems and understanding particle dynamics for complex biological tests.

Keywords:

Aerosol dosimetry Aerosol exposure system characterization In vitro aerosol exposure Vitrocell

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Area of Science:

Aerosol science
In vitro toxicology
Biophysical characterization

Background:

Accurate characterization of in vitro aerosol exposure systems is crucial for interpreting experimental results.
Existing methods for aerosol delivery assessment are often time-consuming, require specialized equipment, or lack real-world experimental applicability.

Purpose of the Study:

To develop and evaluate a novel, sensitive, and robust fluorometry-based method for assessing particle size-specific liquid aerosol delivery.
To provide a more efficient and reliable tool for characterizing in vitro aerosol exposure systems.

Main Methods:

Glycerol aerosols with varying mean particle sizes and narrow distributions, labeled with disodium fluorescein, were generated using a condensation monodisperse aerosol generator.
The generated aerosols were characterized for stability, size distribution, and reproducibility.
Test exposures were conducted in a Vitrocell® 24/48 aerosol exposure system to evaluate the method's sensitivity and feasibility.

Main Results:

The developed fluorometry method demonstrated high sensitivity and robustness in assessing particle size-specific aerosol delivery.
Characterization confirmed the stability and reproducibility of the generated glycerol aerosols.
Feasibility was confirmed through test exposures in a relevant in vitro aerosol exposure system.

Conclusions:

The new fluorometry-based method offers a sensitive, robust, and reproducible approach for evaluating liquid aerosol delivery in in vitro systems.
This method can be applied to confirm computational simulations, characterize exposure systems, and describe aerosol delivery in complex biological test systems.