Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Machines: Problem Solving I01:22

Machines: Problem Solving I

777
A toggle clamp is a mechanical device commonly used for holding and clamping objects in various applications, such as woodworking, metalworking, and assembly operations. Consider a toggle clamp subjected to a force of 200 N at the handle. The vertical clamping force can be calculated, provided the dimensions of the toggle clamp are known.
The toggle clamp system is a machine structure consisting of movable, pin-connected multi-force members that form a stabilized system to transmit forces. The...
777
The Replisome03:01

The Replisome

39.3K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
39.3K
Eccentric Loading01:16

Eccentric Loading

1.1K
Eccentric loading is a crucial concept in the study of structural engineering and mechanics, particularly when analyzing the stability and stress distribution in columns. Unlike centric loading, where the force is applied along the centroidal axis, causing uniform compression, eccentric loading occurs when a force is applied off-center. This off-center application introduces not only direct compressive stress but also bending stress, significantly influencing the column's behavior under...
1.1K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

9.9K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
9.9K
Clamper Circuit01:14

Clamper Circuit

1.1K
A clamper circuit, also known as a DC restorer, represents a specialized variant of the rectifier circuit, notable for its method of taking the output across the diode rather than the capacitor. This configuration lends to several distinctive applications, particularly in handling square wave inputs.
Within this circuit, the diode's orientation prompts the capacitor to charge up to the level of the most negative peak of the input signal. Upon reaching this state, the diode ceases to...
1.1K
Pumped Concrete01:13

Pumped Concrete

466
Concrete in large quantities can be pumped across long distances for placing in inaccessible sites. This system comprises a hopper that receives concrete from a mixer, a pump to propel the concrete, and pipelines that facilitate its delivery.
For direct-acting pumps, the concrete enters the pump via the inlet valve under the action of gravity and suction created by the movement of the piston. This concrete is then forced into the pipeline and out through the outlet valve by the forward movement...
466

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pds5 regulates sister chromatid cohesion by controlling cohesin ATPase activity through the Eco1-Smc3 acetylation pathway.

Nucleic acids research·2026
Same author

TORC2 coordinates MBF-dependent transcription and restrains oxidative stress responses during DNA replication stress in fission yeast.

The Journal of biological chemistry·2026
Same author

Signing protein-protein interaction networks.

Bioinformatics (Oxford, England)·2025
Same author

Mutations and mitotic recombination events in nondividing cells.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Calcium Signaling Is a Universal Carbon Source Signal Transducer and Effects an Ionic Memory of Past Carbon Sources.

International journal of molecular sciences·2025
Same author

Effects of PCNA Stability on the Formation of Mutations.

International journal of molecular sciences·2024
Same journal

Yeasts in the Gastrointestinal Tract.

FEMS yeast research·2026
Same journal

Nutrient sensing and transceptor-mediated metabolic control in yeast.

FEMS yeast research·2026
Same journal

Establishment of a Protoplast Transformation Method in the Oleaginous Yeast Rhodotorula toruloides.

FEMS yeast research·2026
Same journal

The oxidative cost of losing low temperature viability protein 1 [Ltv1]: insights into cellular damage and homeostasis.

FEMS yeast research·2026
Same journal

Systems biology of yeast metabolism.

FEMS yeast research·2026
Same journal

Uncorking wine yeast genomics from grape to glass.

FEMS yeast research·2026
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

Application of Design Aspects in Uniaxial Loading Machine Development
05:23

Application of Design Aspects in Uniaxial Loading Machine Development

Published on: September 19, 2018

6.4K

Alternative clamp loaders/unloaders.

Martin Kupiec1

  • 1Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel martin@post.tau.ac.il.

FEMS Yeast Research
|September 25, 2016
PubMed
Summary
This summary is machine-generated.

Accurate DNA replication and chromatin formation rely on the PCNA protein clamp. Four complexes, including Replication Factor C (RFC), load PCNA onto DNA, ensuring genome stability and proper cell division in yeast.

Keywords:
Ctf18DNA replicationElg1RFCRad24genome stability

More Related Videos

Manufacturing Devices and Instruments for Easier Rat Liver Transplantation
08:02

Manufacturing Devices and Instruments for Easier Rat Liver Transplantation

Published on: May 5, 2013

9.3K
Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Published on: April 4, 2025

1.4K

Related Experiment Videos

Last Updated: Mar 14, 2026

Application of Design Aspects in Uniaxial Loading Machine Development
05:23

Application of Design Aspects in Uniaxial Loading Machine Development

Published on: September 19, 2018

6.4K
Manufacturing Devices and Instruments for Easier Rat Liver Transplantation
08:02

Manufacturing Devices and Instruments for Easier Rat Liver Transplantation

Published on: May 5, 2013

9.3K
Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Published on: April 4, 2025

1.4K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Accurate genome duplication is essential for cell division.
  • Proliferating Cell Nuclear Antigen (PCNA) is a crucial DNA clamp involved in replication and chromatin assembly.
  • Coordinated action of DNA polymerases and accessory proteins is vital for timely and accurate DNA replication.

Purpose of the Study:

  • To summarize current knowledge on the roles of PCNA loading/unloading complexes in yeast.
  • To highlight the functions of Replication Factor C (RFC) and related complexes (Rad24, Ctf18, Elg1) in DNA metabolism.
  • To emphasize the importance of these factors in ensuring genome integrity.

Main Methods:

  • Literature review of studies on PCNA and its associated factors in yeast.
  • Analysis of the functional roles of RFC, Rad24, Ctf18, and Elg1 complexes.
  • Integration of findings on DNA replication, chromatin formation, and sister chromatid segregation.

Main Results:

  • PCNA acts as a sliding clamp, enhancing DNA polymerase processivity and serving as a platform for DNA-interacting proteins.
  • RFC is the primary complex for loading PCNA onto DNA during S-phase.
  • Rad24, Ctf18, and Elg1 form RFC-like complexes with specialized roles in nuclear DNA processing and repair.
  • These factors collectively influence replication rate, accuracy, chromatin re-formation, and chromosome segregation.

Conclusions:

  • PCNA loading factors are essential for maintaining genome stability during DNA replication and cell division in yeast.
  • The coordinated action of RFC and RFC-like complexes ensures accurate DNA replication and proper chromatin dynamics.
  • Understanding these factors provides insights into fundamental mechanisms of genome maintenance.