Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Technique for cellular fluorescence distribution analysis.

R D Robinson1, J E Reeder, L L Wheeless

  • 1Department of Pathology, University of Rochester Medical Center, New York 14642.

Cytometry
|July 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cold as a Therapeutic and Prophylactic Agent.

Texas medical journal (Austin, Tex.)·2023
Same author

Business models and provider satisfaction in in vitro fertilization centers in the USA.

Journal of assisted reproduction and genetics·2018
Same author

An epidemiological study of A. cantonensis in Jamaica subsequent to an outbreak of human cases of eosinophilic meningitis in 2000.

Parasitology·2016
Same author

Gastrointestinal helminths of wild hogs and their potential livestock and public health significance in Jamaica.

Journal of helminthology·2016
Same author

Eryngial (trans-2-dodecenal), a bioactive compound from Eryngium foetidum: its identification, chemical isolation, characterization and comparison with ivermectin in vitro.

Parasitology·2013
Same author

Non-equilibrium phonon generation and detection in microstructure devices.

The Review of scientific instruments·2011
Same journal

Measurement of the distribution of red blood cell deformability using an automated rheoscope.

Cytometry·2002
Same journal

Flow cytometric-based isolation of nucleated erythroid cells during maturation: an approach to cell surface antigen studies.

Cytometry·2002
Same journal

Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients.

Cytometry·2002
Same journal

Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.

Cytometry·2002
Same journal

Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

Cytometry·2002
Same journal

Separation of live cells in different phases of the cell cycle for gene expression analysis.

Cytometry·2002
See all related articles

This study presents efficient algorithms for analyzing cellular fluorescence distributions from multidimensional slit-scan flow cytometry. These methods rapidly extract key features like peaks and nuclear boundaries for real-time data processing.

Area of Science:

  • Biophysical techniques
  • Quantitative cell analysis
  • Flow cytometry

Background:

  • Multidimensional slit-scan flow cytometry generates complex cellular fluorescence distributions.
  • Extracting relevant features from these distributions is crucial for whole cell measurements.
  • Rapid feature extraction is necessary for real-time data processing during acquisition.

Purpose of the Study:

  • To describe two novel algorithms for analyzing cellular fluorescence distributions.
  • To enable rapid, real-time feature extraction for slit-scan flow cytometry.
  • To facilitate accurate whole cell measurements using flow cytometry data.

Main Methods:

  • Development of two algorithms for analyzing slit-scan contours (cellular fluorescence distributions).

Related Experiment Videos

  • Algorithm 1: Efficient counting of local maxima (peaks).
  • Algorithm 2: Robust identification of nuclear boundaries.

    • Main Results:

    • Successfully implemented algorithms for counting peaks and finding nuclear boundaries.
    • Algorithms demonstrated efficiency using simple integer arithmetic.
    • Routines were successfully implemented on multiple microprocessors, confirming broad applicability.

    Conclusions:

    • The developed algorithms are effective for extracting key features from cellular fluorescence distributions.
    • These methods support rapid, real-time data processing in multidimensional slit-scan flow cytometry.
    • The algorithms' efficiency and portability enhance their utility in quantitative cell analysis.