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Updated: Mar 14, 2026

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Published on: June 15, 2017

PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly.

Maryia Trubitsyna¹, Chao-Kuo Liu², Alejandro Salinas²

¹School of Biological Sciences, University of Edinburgh, Roger Land Building, Edinburgh, EH9 3FF, UK. M.Trubitsyna@ed.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|September 28, 2016

Summary

This summary is machine-generated.

PaperClip is a new DNA assembly method allowing multiple sequences to join in any order within a single reaction. This combinatorial DNA assembly bypasses the need for specific oligonucleotides for each construct order.

Keywords:

Agarose gel electrophoresis CPEC Cell extract DNA assembly Ligation Molecular cloning Oligonucleotide annealing PAGE PCR SLiCE Selection Single-pot assembly

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Last Updated: Mar 14, 2026

Protocols for C-Brick DNA Standard Assembly Using Cpf1

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Published on: June 15, 2017

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

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Published on: May 8, 2015

Area of Science:

Molecular Biology
Synthetic Biology
Biotechnology

Background:

Joining DNA sequences is fundamental for molecular biology.
Existing methods for multi-part DNA assembly often require specific oligonucleotides for each desired construct order.

Purpose of the Study:

To introduce PaperClip, a novel method for DNA assembly.
To enable combinatorial assembly of multiple DNA sequences in a single reaction.

Main Methods:

The PaperClip method facilitates the assembly of multiple DNA sequences.
It operates in a combinatorial manner, allowing flexibility in construct design.

Main Results:

PaperClip enables the assembly of a given set of DNA parts in any desired order.
This method streamlines the creation of linear and circular DNA constructs.

Conclusions:

PaperClip offers a versatile and efficient approach to DNA assembly.
It overcomes limitations of previous methods by eliminating the need for order-specific oligonucleotides.