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Improved production of propionic acid using genome shuffling.

Carlos H Luna-Flores1, Robin W Palfreyman1, Jens O Krömer1

  • 1Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Qld, Australia.

Biotechnology Journal
|September 28, 2016
PubMed
Summary

Genome shuffling improved propionic acid production by 25% in Propionibacterium species. This biological production method, key for sustainable chemical manufacturing, was enhanced through random mutagenesis and next-generation sequencing to identify genetic improvements.

Keywords:
Genome shufflingNext generation sequencingPropionibacterium acidipropioniciPropionic acidProtoplast fusion

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Area of Science:

  • Microbiology
  • Biotechnology
  • Metabolic Engineering

Background:

  • Biological propionic acid production offers a sustainable alternative to fossil fuel-derived sources.
  • Propionibacterium species are key producers, but yields are limited by by-product formation.
  • Genetic engineering of high-producing Propionibacterium strains remains a significant challenge.

Purpose of the Study:

  • To enhance propionic acid yield and productivity in Propionibacterium species.
  • To identify genomic changes responsible for improved production through genome shuffling.
  • To overcome limitations in genetically engineering key propionic acid-producing strains.

Main Methods:

  • Utilized genome shuffling on two wild-type Propionibacterium strains.
  • Employed next-generation sequencing to analyze genomic alterations in improved strains.
  • Quantified propionic acid production to assess strain performance.

Main Results:

  • Developed an improved strain exhibiting a 25% increase in propionic acid production compared to wild-type.
  • Next-generation sequencing revealed genomic changes limited to single point mutations and gene duplications.
  • Identified conserved genomic regions as sites of mutation, suggesting gene conversion mechanisms.

Conclusions:

  • Genome shuffling is an effective strategy for improving propionic acid production in Propionibacterium.
  • The observed genetic changes provide insights into the mechanisms underlying strain improvement.
  • This work advances the biological production of propionic acid, a valuable organic acid.