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Related Experiment Video

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A Step by Step Protocol for Subretinal Surgery in Rabbits
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A Step by Step Protocol for Subretinal Surgery in Rabbits.

Sami Al-Nawaiseh1, Fabian Thieltges1, Zengping Liu2

  • 1Department of Ophthalmology, University of Bonn.

Journal of Visualized Experiments : Jove
|September 30, 2016
PubMed
Summary

Researchers developed a novel method for retinal pigment epithelium (RPE) transplantation in rabbits, a promising approach for treating irreversible vision loss from diseases like age-related macular degeneration (AMD). This technique offers a reproducible and effective preclinical model for future human therapies.

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Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Preclinical Research

Background:

  • Age-related macular degeneration (AMD) and retinitis pigmentosa cause irreversible vision loss.
  • Retinal pigment epithelium (RPE) transplantation is a potential therapy to restore vision.
  • Large animal models are crucial for preclinical translation of eye therapies.

Purpose of the Study:

  • To describe a refined method for RPE monolayer transplantation into the subretinal space (SRS) of rabbits.
  • To establish a reproducible and successful large animal model for RPE transplantation studies.
  • To facilitate preclinical evaluation of RPE-based therapies for vision restoration.

Main Methods:

  • Developed a custom shooter for delivering cultured RPE monolayers on scaffolds into the rabbit SRS post-vitrectomy.
  • Refined the surgical technique over 150 procedures across 6 years.
  • Utilized spectral domain optical coherence tomography (SD-OCT) and histology for post-operative assessment.

Main Results:

  • The described method ensures a high success rate and is easy to learn.
  • Rabbits serve as a cost-effective and suitable large eye model for preclinical studies.
  • The technique allows for non-invasive, repetitive in vivo imaging and histological analysis.

Conclusions:

  • The developed RPE transplantation technique in rabbits is a viable and effective preclinical model.
  • This method supports the advancement of RPE-based therapies for blinding retinal diseases.
  • Rabbit models offer a practical alternative for preclinical research in ophthalmology.