Related Experiment Video
Updated: Jun 19, 2026

Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films
Published on: November 9, 2015
Diffusive gradients in thin films measurement of sulfur stable isotope variations in labile soil sulfate
Ondrej Hanousek1,2, Jakob Santner3,4, Sean Mason5
1Department of Chemistry - VIRIS Laboratory, University of Natural Resources and Life Sciences Vienna, Konrad-Lorenz-Strasse 24, 3430, Tulln, Austria.
Abstract:
A diffusive gradient in thin films (DGT) technique, based on a strongly basic anion exchange resin (Amberlite IRA-400), was successfully tested for 34S/32S analysis in labile soil sulfate. Separation of matrix elements (Na, K, and Ca) that potentially cause non-spectral interferences in 34S/32S analysis by MC ICP-MS (multi-collector inductively coupled plasma-mass spectrometry) during sampling of sulfate was demonstrated. No isotopic fractionation caused by diffusion or elution of sulfate was observed below a resin gel disc loading of ≤79 μg S. Above this threshold, fractionation towards 34S was observed. The method was applied to 11 different topsoils and one mineral soil profile (0-100 cm depth) and compared with soil sulfate extraction by water. The S amount and isotopic ratio in DGT-S and water-extractable sulfate correlated significantly (r 2 = 0.89 and r 2 = 0.74 for the 11 topsoils, respectively). The systematically lower 34S/32S isotope ratios of the DGT-S were ascribed to mineralization of organic S.
More Related Videos
Related Concept Videos
Sulfur Assimilation
Microbes and the Sulfur Cycle

