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Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Chromatography: Introduction01:10

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Gas Chromatography: Types of Columns and Stationary Phases01:17

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Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
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Types Of Column Chromatography01:29

Types Of Column Chromatography

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
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Using a box instead of a column for process chromatography.

Raja Ghosh1

  • 1Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L7, Canada.

Journal of Chromatography. A
|October 1, 2016
PubMed
Summary
This summary is machine-generated.

Chromatography boxes offer a novel solution for process-scale purification challenges. These devices demonstrate superior flow distribution and peak resolution compared to traditional columns, enhancing biopharmaceutical manufacturing.

Keywords:
BioseparationChromatography boxFlow distributionPacked-bedProcess chromatography

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Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Analytical Chemistry

Background:

  • Process-scale chromatography, vital for biopharmaceutical purification, often uses short columns.
  • Non-uniform flow in these columns leads to poor peak resolution, impacting purity, recovery, and productivity.
  • Existing solutions focus on improving column header design.

Purpose of the Study:

  • To introduce and evaluate a novel chromatography box device as an alternative to traditional columns for process-scale chromatography.
  • To compare the performance of chromatography boxes against equivalent columns in terms of flow distribution and separation efficiency.

Main Methods:

  • The study utilized chromatography box devices based on laterally-fed membrane chromatography (LFMC) design.
  • Performance was assessed by comparing chromatography boxes with equivalent packed-bed columns.
  • Key performance indicators included peak sharpness, asymmetry, theoretical plates per meter, and resolution of protein separations.

Main Results:

  • Chromatography box devices exhibited improved flow distribution compared to conventional columns.
  • Enhanced peak sharpness and symmetry were observed with the chromatography box devices.
  • Higher theoretical plates per meter and better peak resolution were achieved using chromatography boxes.

Conclusions:

  • Chromatography boxes represent a promising alternative to traditional columns for process-scale chromatography.
  • The superior performance of chromatography boxes in flow distribution and separation efficiency can benefit biopharmaceutical purification.
  • Further development and application of chromatography box technology are warranted for industrial use.