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A Fast Method for DEFB1 - 44C/G SNP Genotyping in Brazilian Patients with Periodontitis
Renata Cimőes1, Rafael Rafael Amorim Cavalcanti de Siqueira2, Sergio Crovella1
1PhD, Federal University of Pernambuco, Recife, Brazil.
Aim:
Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparison to healthy controls.
Material And Methods:
We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients.
Results:
The results indicate that there are no differences in distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently.
Conclusion:
Further studies are necessary in order to investigate the role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.
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