Isothermal Amplification Strategies for Detection in Microfluidic Devices
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Updated: Mar 14, 2026
Thermal Measurement Techniques in Analytical Microfluidic Devices
Published on: June 3, 2015
Saheli Sarkar1, Pooja Sabhachandani1, Tania Konry1
1Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
Abstract:
Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity. Immuno-RCA, the specific detection of proteins via antigen-antibody recognition, has been miniaturized for microfluidic platforms to reduce reagent and sample consumption, accelerate reaction kinetics, and enhance the sensitivity and specificity of detection.
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