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Related Experiment Video

Updated: Mar 14, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

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Published on: May 21, 2020

Transcriptome Dynamics during Maize Endosperm Development.

Jianzhou Qu¹, Chuang Ma², Jiaojiao Feng¹

¹The Key Laboratory of Biology and Genetics Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.

|October 4, 2016

Summary

Maize endosperm development involves over 11,000 genes with alternative splicing and 7,633 differentially expressed genes, including transcription factors. This study reveals key molecular processes during early seed development.

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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

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Published on: March 5, 2017

Area of Science:

Plant Molecular Biology
Genomics
Seed Development

Background:

The maize endosperm is crucial for seed weight and quality.
Comprehensive genome-wide transcriptome analysis of maize endosperm development is lacking.

Purpose of the Study:

To investigate genome-wide transcriptome patterns during maize endosperm development.
To identify alternative splicing events and differentially expressed genes, including transcription factors, across four developmental stages.

Main Methods:

High-throughput RNA sequencing (RNA-seq) of maize endosperm.
Analysis of transcriptome at 5, 10, 15, and 20 days after pollination (DAP).

Main Results:

Over 11,000 protein-coding genes exhibited alternative splicing (AS) events.
7,633 genes, including 473 transcription factors (TFs) from 50 families, were differentially expressed.
Stage-specific TF activities were identified, suggesting distinct roles in early endosperm development.

Conclusions:

This study provides comprehensive insights into transcriptome dynamics during maize endosperm development.
Alternative splicing and differential gene expression, particularly involving TFs, are critical for early seed development.