Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cellular quiescence uncouples the proteome from the transcriptome in neural stem cells.

The EMBO journal·2026
Same author

Differential chromatin accessibility response to retinoic acid in neuroblastoma with ATRX in-frame-deletions versus ATRX loss-of-function.

Neoplasia (New York, N.Y.)·2025
Same author

Long-lived adult-born hippocampal neurons promote successful cognitive aging.

Molecular psychiatry·2025
Same author

Sequential transcriptional programs underpin activation of hippocampal stem cells.

Science advances·2025
Same author

Nociceptin/OrphaninFQ Receptor Modulates the Maturation of Adult-Born Neurons in the Mouse Dentate Gyrus Under Physiological Conditions and in a Chronic Stress Model.

Molecular neurobiology·2025
Same author

BCL11A intellectual developmental disorder: defining the clinical spectrum and genotype-phenotype correlations.

European journal of human genetics : EJHG·2024
Same journal

Simultaneous Ca<sup>2+</sup> Imaging and Optogenetic Stimulation of Cortical Astrocytes in Adult Murine Brain Slices.

Current protocols in neuroscience·2020
Same journal

Automated Two-Chamber Operon ID/ED Task for Mice.

Current protocols in neuroscience·2020
Same journal

A Guide to Fluorescence Lifetime Microscopy and Förster's Resonance Energy Transfer in Neuroscience.

Current protocols in neuroscience·2020
Same journal

Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research.

Current protocols in neuroscience·2020
Same journal

Recombinant Antibodies in Basic Neuroscience Research.

Current protocols in neuroscience·2020
Same journal

Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model.

Current protocols in neuroscience·2020
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
13:33

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Published on: July 25, 2017

11.8K

Cerebral Cortex Electroporation to Study Projection Neuron Migration.

Emilie Pacary1,2, François Guillemot3

  • 1INSERM U1215, Neurocentre Magendie, Bordeaux, France.

Current Protocols in Neuroscience
|October 4, 2016
PubMed
Summary
This summary is machine-generated.

Brain electroporation is a powerful technique for studying neuronal development, particularly the radial migration of projection neurons in the embryonic cerebral cortex. This method aids in understanding normal brain formation and neurodevelopmental disorders.

Keywords:
cerebral cortexelectroporationmigrationneuron

More Related Videos

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
10:45

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Published on: June 14, 2020

8.0K
Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex
10:12

Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex

Published on: May 18, 2012

12.3K

Related Experiment Videos

Last Updated: Mar 14, 2026

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
13:33

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Published on: July 25, 2017

11.8K
Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
10:45

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Published on: June 14, 2020

8.0K
Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex
10:12

Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex

Published on: May 18, 2012

12.3K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Neuronal migration is crucial for proper cerebral cortex formation.
  • Defects in neuronal migration are linked to neurodevelopmental disorders.
  • Electroporation offers a precise method to study these processes.

Purpose of the Study:

  • To detail protocols for in utero and ex vivo electroporation.
  • To investigate the radial migration of projection neurons in the embryonic cerebral cortex.
  • To facilitate the study of molecular mechanisms controlling neuronal migration.

Main Methods:

  • In utero electroporation of progenitor cells in the embryonic cerebral cortex.
  • Ex vivo electroporation of progenitor cells in the embryonic cerebral cortex.
  • Utilizing electroporation to label and track migrating neurons.

Main Results:

  • Established detailed protocols for brain electroporation.
  • Enabled in-depth analysis of radial migration steps.
  • Provided a foundation for characterizing molecular control of migration.

Conclusions:

  • Brain electroporation is an effective method for studying neuronal migration.
  • Understanding neuronal migration is key to both normal brain development and disease.
  • The described protocols support further research into neurodevelopmental processes.