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Related Experiment Video

Updated: Mar 14, 2026

Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice
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Imaging hemodynamic response after ischemic stroke in mouse cortex using visible-light optical coherence tomography.

Siyu Chen1, Qi Liu2, Xiao Shu3

  • 1Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA; Both authors contributed equally to this work.

Biomedical Optics Express
|October 5, 2016
PubMed
Summary

Visible-light optical coherence tomography (Vis-OCT) improves blood oxygen saturation mapping in scattering tissues like the brain. A new dual-depth strategy enhances accuracy for monitoring microvascular changes during stroke.

Keywords:
(110.4500) Optical coherence tomography(170.1460) Blood gas monitoring(170.2655) Functional monitoring and imaging(170.6480) Spectroscopy, speckle

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Area of Science:

  • Biomedical Optics
  • Neuroimaging
  • Microcirculation Research

Background:

  • Visible-light optical coherence tomography (Vis-OCT) offers high-resolution imaging of microvasculature and hemodynamic responses.
  • Accurate blood oxygen saturation (sO2) mapping in highly scattering tissues, such as the brain cortex, remains a challenge.
  • Tissue scattering significantly degrades the precision of intrinsic optical absorption-based oximetry.

Purpose of the Study:

  • To develop and validate a novel strategy for improving the accuracy of Vis-OCT oximetry in scattering brain tissue.
  • To minimize the impact of tissue scattering on sO2 measurements using Vis-OCT.
  • To monitor microvascular and hemodynamic changes in the mouse cortex following induced focal ischemia.

Main Methods:

  • Formulation and implementation of a dual-depth sampling and normalization strategy for Vis-OCT.
  • Application of the developed method to image hemodynamic responses in the mouse cortex after focal photothrombosis.
  • Analysis of vessel diameter changes and sO2 levels in the penumbra region.

Main Results:

  • The dual-depth strategy effectively minimized detrimental effects of tissue scattering on Vis-OCT oximetry.
  • Observed significant vessel dilatation in the penumbra region, negatively correlated with original vessel diameter.
  • Demonstrated a drop in blood oxygen saturation (sO2) in the penumbra region's vessels below normal levels post-ischemia.

Conclusions:

  • The novel dual-depth sampling and normalization strategy enhances the accuracy of Vis-OCT oximetry in scattering brain tissue.
  • Vis-OCT can effectively monitor microvascular and hemodynamic alterations, including vessel dilation and deoxygenation, during ischemic events.
  • This technique provides valuable insights into the pathophysiology of stroke at the microvascular level.