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Design of a fiber-optic multiphoton microscopy handheld probe
Yuan Zhao1, Mingyu Sheng1, Lin Huang2
1Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, V6T 1Z4, Canada; School of Engineering Science, Simon Fraser University, Burnaby, V5A 1S6, Canada.
Biomedical Optics Express
|October 5, 2016
Summary
Researchers created a handheld fiber-optic multiphoton microscopy (MPM) probe. This advanced system offers high resolution for biological imaging, with potential for further improvement using a custom objective module.
Area of Science:
- Optics
- Biomedical Engineering
- Microscopy
Background:
- Fiber-optic multiphoton microscopy (MPM) enables in-vivo imaging.
- Handheld probes are desirable for flexible microscopy applications.
- Optimizing objective lens design is crucial for high-resolution MPM.
Purpose of the Study:
- To present the optical design and analysis of a handheld MPM probe.
- To compare the performance of commercial and custom objective modules for MPM.
- To evaluate the imaging capabilities of the developed handheld MPM system.
Main Methods:
- Detailed optical design and Zemax simulation of objective modules.
- Wavefront error, field curvature, astigmatism, and F-θ error analysis.
- Experimental implementation and resolution measurement using commercial objectives.
Main Results:
- Tolerance analysis predicted focal spot sizes of 1.13, 1.19, and 0.83 µm.
- Experimental lateral resolution measurements were 1.22 µm and 1.3 µm, matching predictions.
- Successful MPM imaging was demonstrated on leaf, fish scale, and rat tail tendon.
Conclusions:
- The developed fiber-optic handheld MPM probe achieves high resolution.
- A custom objective module shows potential for further resolution enhancement.
- The system is suitable for various biological imaging applications.
