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Published on: October 17, 2016
Design of a fiber-optic multiphoton microscopy handheld probe
Yuan Zhao1, Mingyu Sheng1, Lin Huang2
1Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, V6T 1Z4, Canada; School of Engineering Science, Simon Fraser University, Burnaby, V5A 1S6, Canada.
Abstract:
We have developed a fiber-optic multiphoton microscopy (MPM) system with handheld probe using femtosecond fiber laser. Here we present the detailed optical design and analysis of the handheld probe. The optical systems using Lightpath 352140 and 352150 as objective lens were analyzed. A custom objective module that includes Lightpath 355392 and two customized corrective lenses was designed. Their performances were compared by wavefront error, field curvature, astigmatism, F-θ error, and tolerance in Zemax simulation. Tolerance analysis predicted the focal spot size to be 1.13, 1.19 and 0.83 µm, respectively. Lightpath 352140 and 352150 were implemented in experiment and the measured lateral resolution was 1.22 and 1.3 µm, respectively, which matched with the prediction. MPM imaging by the handheld probe were conducted on leaf, fish scale and rat tail tendon. The MPM resolution can potentially be improved by the custom objective module.
