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Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
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Biomaterials in siRNA Delivery: A Comprehensive Review
William Ho1, Xue-Qing Zhang1, Xiaoyang Xu1
1Department of Chemical, Biological and Pharmaceutical Engineering, Newark School of Engineering, New Jersey Institute of Technology, Newark, NJ, 07102, USA.
Advanced Healthcare Materials
|October 5, 2016
Summary
RNA interference (RNAi) therapeutics offer promise for diseases like Ebola and cancer. This review covers challenges and advancements in small interfering RNA (siRNA) delivery systems for effective clinical use.
Area of Science:
- Biotechnology
- Molecular Biology
- Therapeutics
Background:
- RNA interference (RNAi) is a powerful gene-silencing mechanism with therapeutic potential for diseases like cancer and Ebola.
- Small interfering RNAs (siRNAs) face challenges including large size, structural instability, and negative charge, hindering clinical application.
- Developing safe and effective delivery vehicles is crucial for advancing RNAi therapeutics.
Purpose of the Study:
- To review the in vivo barriers that impede siRNA delivery systems.
- To discuss the progress and design of advanced siRNA delivery systems.
- To highlight clinically relevant delivery strategies for RNAi therapeutics.
Main Methods:
- Review of existing literature on siRNA delivery systems.
- Analysis of challenges in in vivo siRNA delivery.
- Discussion of liposomal, polymeric, and siRNA conjugate delivery systems.
Main Results:
- Identified key in vivo barriers for siRNA delivery.
- Detailed progress in developing advanced siRNA delivery systems.
- Highlighted successful strategies in liposomal, polymeric, and conjugate delivery.
Conclusions:
- Effective siRNA delivery systems are critical for realizing the potential of RNAi therapeutics.
- Significant advancements have been made in overcoming delivery challenges.
- Liposomal, polymeric, and conjugate systems represent promising clinically advanced strategies.
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