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Related Experiment Video

Updated: Mar 14, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

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Extracting quantitative information from single-molecule super-resolution imaging data with LAMA - LocAlization

Sebastian Malkusch¹, Mike Heilemann¹

¹Single Molecule Biophysics, Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Germany.

Scientific Reports

|October 6, 2016

Summary

This summary is machine-generated.

The LocAlization Microscopy Analyzer (LAMA) software provides quantitative analysis for super-resolution microscopy data. It helps researchers characterize cellular structures and protein organization at the nanoscale.

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Last Updated: Mar 14, 2026

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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

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Published on: April 19, 2019

Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Super-resolution fluorescence microscopy offers nanoscale insights into protein organization within cells.
Advanced image analysis tools are crucial for maximizing information extraction from such data.

Purpose of the Study:

Introduce the LocAlization Microscopy Analyzer (LAMA), a software for quantitative analysis of super-resolution imaging data.
Enable detailed characterization of cellular structures and protein localization.

Main Methods:

Development of the LocAlization Microscopy Analyzer (LAMA) software.
Application of LAMA to extract quantitative information from single-molecule super-resolution data.

Main Results:

LAMA characterizes cellular structures by size, shape, intensity, and distribution.
The software quantifies the degree of colocalization between different cellular structures.
LAMA provides direct access to individual analysis of super-resolution data.

Conclusions:

LAMA is a comprehensive, freely available, and platform-independent tool for super-resolution microscopy data analysis.
The software facilitates deeper understanding of nanoscale protein organization and cellular structures.