Related Experiment Video
Updated: Mar 14, 2026
06:12
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
2.1K
A compact imaging spectroscopic system for biomolecular detections on plasmonic chips
Shu-Cheng Lo1, En-Hung Lin2, Pei-Kuen Wei2
1Department of Applied Materials and Optoelectronics Engineering, National Chi Nan University, Nantou 54561, Taiwan. wstsai@ncnu.edu.tw.
The Analyst
|October 6, 2016
Summary
We developed a compact, cost-effective imaging spectroscopic system for high-throughput detection of biomolecular interactions. This label-free system achieves 1 nm spectral resolution, enabling sensitive and real-time screening of protein binding on plasmonic chips.
Area of Science:
- Biophotonics
- Spectroscopy
- Plasmonics
Background:
- Plasmonic chips are crucial for detecting biomolecular interactions.
- Existing spectroscopic systems can be bulky and expensive.
- High-throughput screening is essential for drug discovery and diagnostics.
Purpose of the Study:
- To develop a compact, cost-effective imaging spectroscopic system.
- To enable high-throughput, label-free detection of biomolecular interactions.
- To achieve high spectral resolution for sensitive measurements.
Main Methods:
- Fabrication of curved gratings and plasmonic chips on flexible substrates using gas-assisted thermal embossing.
- Integration of a fiber-coupled broadband light source and camera.
- Utilizing a 1D scanning stage for high-throughput analysis.
Main Results:
- Achieved spectral resolution within 1 nm for sensing environmental index and protein binding.
- Demonstrated sensitivity comparable to commercial spectrometers.
- Detected wavelength shifts of 1-9 nm for antigen-antibody binding across various concentrations.
Conclusions:
- The developed system offers a simple, mass-productive, and cost-effective solution.
- Enables real-time, label-free, highly sensitive, and high-throughput screening of biomolecular interactions.
- The compact design and flexible fabrication hold promise for portable diagnostic devices.
