Related Experiment Video
Updated: Mar 14, 2026
11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
9.9K
Epithelial-mesenchymal transition in pediatric nephropathies
Laura Penna Rocha1, Samuel Cavalcante Xavier1, Fernanda Rodrigues Helmo1
1Department of General Pathology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
Pathology, Research and Practice
|October 7, 2016
Summary
Epithelial-mesenchymal transition (EMT) markers like TGF-β3, fibronectin, α-SMA, and vimentin were elevated in pediatric kidney diseases. These markers correlate with kidney function impairment, suggesting their potential as diagnostic indicators.
Area of Science:
- Pediatric Nephrology
- Cell Biology
- Renal Pathology
Background:
- Epithelial-mesenchymal transition (EMT) involves cellular changes and is implicated in kidney disease pathogenesis.
- Understanding EMT markers in pediatric nephropathies is crucial for diagnosis and management.
Purpose of the Study:
- To investigate the expression of EMT inducers and markers in common pediatric kidney diseases.
- To correlate marker expression with renal function parameters.
Main Methods:
- Immunohistochemical analysis of TGF-β3, fibronectin, α-SMA, and vimentin in 59 pediatric patients across 6 nephropathy groups and a control group.
- Recording of urea and creatinine levels.
Main Results:
- Elevated glomerular TGF-β3 in Lupus Nephritis and Acute Diffuse Glomerulonephritis (ADGN).
- Increased glomerular fibronectin in Podocytopathy, Lupus Nephritis, ADGN, and Membranous Glomerulopathy.
- Higher α-SMA in tubulointerstitial and glomerular compartments in ADGN and Membranous Glomerulopathy.
- Elevated glomerular vimentin in ADGN compared to other conditions.
- Positive correlations between fibronectin, α-SMA, vimentin, and renal function markers (urea, creatinine).
Conclusions:
- The studied EMT markers show altered expression in various pediatric nephropathies.
- These markers may serve as potential indicators of renal functional impairment in pediatric kidney diseases.
Related Concept Videos
01:10Nephrons
8.1K
The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
8.1K
01:24Nephrotic Syndrome I : Introduction
821
Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of...
821
01:29Acute Kidney Injury II: Pathophysiology
1.6K
Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
1.6K
02:30Metastasis
6.8K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.8K
01:19Cadherins in Tissue Organization
4.4K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
4.4K
01:24Pharmacokinetics in Pediatric Patients: Drug Metabolism
309
In pediatric care, understanding the nuances of hepatic drug metabolism is crucial, as it significantly differs from that of adults. This divergence is primarily due to the developmental stage of drug-metabolizing enzymes, which affects how medications are processed in the body. In neonates, for instance, the activity of Phase I enzymes—critical for the initial breakdown of drugs—is markedly reduced, functioning at just 20–40% of the levels seen in adults. This reduction poses...
309