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Related Experiment Video

Updated: Mar 14, 2026

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Single-cell intracellular nano-pH probes.

Rıfat Emrah Özel¹, Akshar Lohith¹, Wai Han Mak¹

¹Biomolecular Engineering Department, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.

|October 7, 2016

Summary

This summary is machine-generated.

This study introduces a novel nano-pH probe for precise, real-time intracellular pH measurements in single cells. This technology aids in understanding cellular heterogeneity and advancing personalized medicine.

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Last Updated: Mar 14, 2026

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Cell Biology

Background:

Cellular heterogeneity in intracellular pH is crucial for understanding diseases like cancer.
Existing technologies struggle with quantitative, real-time single-cell pH measurements.

Purpose of the Study:

To develop a novel nanopipette-based sensor for accurate single-cell intracellular pH monitoring.
To overcome limitations in current pH measurement technologies for individual cells.

Main Methods:

Development of a nano-pH probe by functionalizing quartz nanopipettes with chitosan.
Characterization of the probe's dynamic pH range (2.6-10.7) and sensitivity (0.09 pH units).
Application of the probe for real-time intracellular pH measurements in various human cell lines (fibroblasts, HeLa, MDA-MB-231, MCF-7).

Main Results:

The chitosan-functionalized nanoprobe achieved high sensitivity and a broad dynamic pH range.
Successful real-time, continuous intracellular pH monitoring was demonstrated in both cancerous and non-cancerous cells.
The sensor captured cellular pH responses to pharmaceutical interventions.

Conclusions:

Chitosan-functionalized nanopipettes offer a powerful tool for high-resolution single-cell pH sensing.
This technology has significant potential for clinical applications, including personalized medicine and cancer research.
The nano-tool enables detailed study of cellular heterogeneity and dynamic pH changes.