Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

6.5K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
6.5K
Antianginal Drugs: Nitrates and β-Blockers01:16

Antianginal Drugs: Nitrates and β-Blockers

1.8K
In cardiovascular health, antianginal drugs combat angina pectoris — a condition marked by chest pain owing to diminished blood flow to the heart.
Organic nitrates,  such as nitroglycerin, play a pivotal role. Once metabolized, they liberate nitric oxide, a molecular marvel. Nitric oxide triggers guanylyl cyclase and augments cGMP production. This biochemical cascade orchestrates the relaxation of vascular smooth muscles, ushering in vasodilation and enhancing coronary blood flow....
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extremely large solid pseudopapillary neoplasm of the pancreas.

Clinical journal of gastroenterology·2026
Same author

RNA duplex formation and competing endogenous RNA, proposed as mechanisms in regulating expression of natural antisense transcripts- from hypotheses to potential therapeutic applications.

Frontiers in molecular biosciences·2026
Same author

Pharmacokinetic and pharmacogenomic profile of capecitabine and oxaliplatin in a patient with gastric cancer undergoing hemodialysis: a case report.

Cancer chemotherapy and pharmacology·2026
Same author

Exploratory Evaluation of Multidisciplinary Collaboration in Developing a Community-Based Heart Failure Care System.

Circulation reports·2026
Same author

Signal Detection Analysis of Hypnotic-induced Respiratory Depression.

In vivo (Athens, Greece)·2026
Same author

Diterpenes in Aralia cordata Rhizomes Suppress the Expression of Proinflammatory and Extracellular Matrix Degradation-Related Genes in ATDC5 Cells.

Biological & pharmaceutical bulletin·2026

Related Experiment Video

Updated: Mar 14, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.6K

Lignans from guaiac resin decrease nitric oxide production in interleukin 1β-treated hepatocytes.

Yuki Nakano1, Masaaki Nasu2, Mana Kano1

  • 1Department of Biomedical Sciences, College of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577, Japan.

Journal of Natural Medicines
|October 7, 2016
PubMed
Summary

Guaiac resin exhibits anti-inflammatory properties, with six purified lignans significantly reducing nitric oxide (NO) production in liver cells. These lignans are likely responsible for the resin's therapeutic effects.

Keywords:
Anti-inflammatory effectGuaiac resinGuaiacum officinaleHepatocyteLignanNitric oxide

More Related Videos

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

10.1K
Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
07:19

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements

Published on: July 29, 2021

2.8K

Related Experiment Videos

Last Updated: Mar 14, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.6K
En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

10.1K
Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
07:19

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements

Published on: July 29, 2021

2.8K

Area of Science:

  • Phytochemistry
  • Pharmacology
  • Immunology

Background:

  • Guaiac resin, derived from the *Guaiacum* species, is traditionally recognized for potential anti-inflammatory effects.
  • Nitric oxide (NO) production is a key indicator of inflammation, often modulated by anti-inflammatory agents.

Purpose of the Study:

  • To isolate and identify lignans from guaiac resin.
  • To evaluate the anti-inflammatory potential of these isolated lignans by assessing their effect on NO production.

Main Methods:

  • Lignans were purified from guaiac resin using rat hepatocytes stimulated with interleukin-1β (IL-1β).
  • Nitric oxide (NO) production was monitored as a measure of anti-inflammatory activity.
  • Six lignans were identified: dehydroguaiaretic acid (1), (+)-trans-1,2-dihydrodehydroguaiaretic acid (2), furoguaiaoxidin (3), meso-dihydroguaiaretic acid (4), furoguaiacin (5), and nectandrin B (6).

Main Results:

  • Six lignans were successfully isolated and identified from guaiac resin.
  • Compounds 1, 2, and 3 were reported as novel isolations from guaiac resin or natural sources.
  • All six lignans (1-6) demonstrated significant suppression of NO production in IL-1β-treated hepatocytes.

Conclusions:

  • The isolated lignans possess significant NO-suppressing activity, supporting the anti-inflammatory effects of guaiac resin.
  • These lignans are likely the active compounds mediating the anti-inflammatory properties of guaiac resin.
  • This study provides a scientific basis for the traditional use of guaiac resin as an anti-inflammatory agent.