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Updated: Mar 14, 2026

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Published on: December 20, 2017
A bioorthogonal approach for imaging the binding between Dasatinib and its target proteins inside living cells
Young-Rang Kim1, Young Hye Kim2, Sung Woo Kim1
1Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Daejeon 34133, Korea. kblee@kbsi.re.kr.
Abstract:
Herein, we present a simple readout of the binding between a chemical drug and its target proteins in the cytoplasm by using a two-step bioorthogonal labeling method combined with spatially-localized expression of proteins. Dasatinib was modified with trans-cyclooctene (TCO), and its cytoplasmic target kinases were expressed in intracellular compartments, such as endosomes and F-actins. After bioorthogonal labeling, the colocalization between Dasatinib and its target proteins was observed in intracellular compartments.
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