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Published on: March 22, 2020
A fluorescent ELISA based on the enzyme-triggered synthesis of poly(thymine)-templated copper nanoparticles
Jian Sun1, Tao Hu2, Xiaolong Xu1
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. xryang@ciac.ac.cn.
Abstract:
Inspired by the ALP-triggered hydrolysis of the substrate ascorbic acid 2-phosphate as well as the product ascorbate-controlled generation of polyT-CuNPs with bright red fluorescence, we have developed a novel, selective and sensitive fluorescence turn-on assay for ALP activity sensing. Based on the conventional ELISA platform and commercially available antibody-ALP conjugates, an unconventional fluorescent ELISA system has been rationally developed and successfully applied in the quantitative measurement of model antigen proteins using fluorescence spectroscopy and a naked-eye readout under ultraviolet light.
