Related Experiment Video
Updated: Mar 14, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Published on: July 9, 2021
Progress toward reaction monitoring at variable temperatures: a new stopped-flow NMR probe design
1Department of Chemistry, University of Wisconsin, Madison, WI, United States.
Abstract:
A stopped-flow NMR probe is described that enables fast flow rates, short transfer times, and equilibration of the reactant magnetization and temperature prior to reaction. The capabilities of the probe are demonstrated by monitoring the polymerization of lactide as catalyzed by the air-sensitive catalyst 1,3-dimesitylimidazol-2-ylidene (IMes) over the temperature range of -30 to 40 °C. The incorporation of stopped-flow capabilities into an NMR probe permits the rich information content of NMR to be accessed during the first few seconds of a fast reaction. Copyright © 2016 John Wiley & Sons, Ltd.
More Related Videos
Related Concept Videos
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
Effect of Temperature Change on Reaction Rate
Atomic Spectroscopy: Effects of Temperature
At thermal equilibrium, the relative populations of excited and ground state atoms can be estimated using the Maxwell–Boltzmann distribution. For example, an increase in temperature...
Temperature Dependence on Reaction Rate
Atoms, molecules, or ions must collide before they can react with each other. Atoms must be close together to form chemical bonds. This premise is the basis for a theory that explains many observations regarding chemical kinetics, including factors affecting reaction rates.
The collision theory is based on the postulates that (i) the reaction rate is proportional to the rate of reactant collisions, (ii) the reacting species collide in an orientation allowing contact between...
NMR Spectrometers: Resolution and Error Correction
Chemical Shift: Internal References and Solvent Effects
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...

