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An LC-MS/MS method for multi-mycotoxin quantification in cow milk.

Myra E Flores-Flores1, Elena González-Peñas1

  • 1Organic and Pharmaceutical Chemistry Department, Faculty of Pharmacy and Nutrition, University of Navarra, C/ Irunlarrea 1, 31008 Pamplona, Navarra, Spain.

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|October 11, 2016
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Summary

This study presents a validated liquid chromatography-mass spectrometry method for quantifying 15 mycotoxins in cow milk. The method accurately detects common and rare mycotoxins, ensuring milk safety.

Keywords:
AflatoxinFumonisinLC–MS/MSMilkMycotoxinOchratoxin

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Area of Science:

  • Food Chemistry
  • Analytical Chemistry
  • Toxicology

Background:

  • Mycotoxins in cow milk pose a significant risk to human health.
  • Accurate and simultaneous quantification of multiple mycotoxins is crucial for food safety monitoring.
  • Existing methods may not cover a broad spectrum of mycotoxins, including less frequently studied ones.

Purpose of the Study:

  • To develop and validate a simultaneous method for quantifying 15 mycotoxins in cow milk.
  • To assess the method's performance, including limits of detection, quantification, accuracy, and recovery.
  • To enable the detection of both major and less common mycotoxins in milk.

Main Methods:

  • Simultaneous quantification of 15 mycotoxins using liquid chromatography-mass spectrometry (LC-MS).
  • Extraction using acidified acetonitrile followed by a sodium acetate cleanup step.
  • Comprehensive method validation including LOD, LOQ, linearity, precision, accuracy, recovery, matrix effect, and stability.

Main Results:

  • Achieved low limits of detection (LOD) for 15 mycotoxins, ranging from 0.02 to 10.14 ng/mL.
  • Demonstrated high recovery rates (82.6–94.4%) for most mycotoxins, with lower recovery for fumonisins (42.1–64.6%).
  • Observed matrix effects (25.5–96.8%) for all mycotoxins, particularly deepoxy-deoxynivalenol, zearalenone, and sterigmatocystin.

Conclusions:

  • The validated LC-MS method reliably quantifies 15 mycotoxins in cow milk.
  • The method is suitable for monitoring common mycotoxins and those less frequently studied, such as fumonisins, sterigmatocystin, and ochratoxin B.
  • This analytical approach enhances the safety assessment of milk products by covering a wider range of potential contaminants.