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Related Experiment Video

Updated: Mar 13, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

High throughput solid phase microextraction: A new alternative for analysis of cellular lipidome?

Afsoon Pajand Birjandi¹, Barbara Bojko¹, Zhibin Ning²

¹Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|October 11, 2016

Summary

A novel solid-phase microextraction (SPME) method offers a more diverse lipid profile analysis for cell cultures compared to traditional methods. This SPME technique provides precise and sensitive lipidomic profiling, aiding in understanding cellular responses to stimuli.

Keywords:

Automation Cell line study Lipidomics Solid phase microextraction

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A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

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Last Updated: Mar 13, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

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Published on: March 8, 2020

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Area of Science:

Analytical Chemistry
Lipidomics
Cell Biology

Background:

Lipidomic studies are crucial for understanding cellular functions and responses to stimuli.
Conventional methods like Bligh & Dyer have limitations in lipid diversity and matrix effects.
Monitoring lipid profile changes in cell line cultures requires sensitive and comprehensive analytical techniques.

Purpose of the Study:

To introduce and validate a novel solid-phase microextraction (SPME) method for untargeted lipidomic analysis of cell line cultures.
To assess the feasibility of SPME for monitoring lipid profile alterations following external stimuli.
To compare the performance of the SPME method against the conventional Bligh & Dyer technique.

Main Methods:

Development and application of a new SPME method for lipid extraction from cell line cultures.
Utilized human hepatocellular carcinoma (HCC) cell line as a model system.
Comparison of lipid profiles between control cells and cells treated with polyunsaturated fatty acid (20:5) using both SPME and Bligh & Dyer methods.

Main Results:

The SPME method identified 77 significantly different lipid species between control and treated cells, with 63 up-regulated in treated cells.
SPME revealed greater diversity in lipid classes and subclasses (e.g., LPC, sphingomyelins, ceramides, prenol lipids) compared to Bligh & Dyer.
SPME demonstrated method precision within 5-18% RSD and no significant matrix effects, unlike Bligh & Dyer which showed ionization suppression/enhancement.

Conclusions:

The novel SPME method is a feasible and advantageous technique for untargeted lipidomic studies of cell cultures.
SPME provides a more comprehensive lipid profile and superior analytical performance over the Bligh & Dyer method.
This SPME approach enables effective monitoring of cellular lipid changes in response to external stimuli.