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Published on: September 25, 2018
A fuel-limited isothermal DNA machine for the sensitive detection of cellular deoxyribonucleoside triphosphates
Jiantong Dong1, Tongbo Wu1, Yu Xiao2
1Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.
Abstract:
A fuel-limited isothermal DNA machine has been built for the sensitive fluorescence detection of cellular deoxyribonucleoside triphosphates (dNTPs) at the fmol level, which greatly reduces the required sample cell number. Upon the input of the limiting target dNTP, the machine runs automatically at 37 °C without the need for higher temperature.

