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Updated: Mar 13, 2026

Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons

Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons

Published on: December 10, 2009

Pronuclear Injection-Based Targeted Transgenesis.

Samantha L P Schilit¹, Masato Ohtsuka^2,3, Rolen M Quadros⁴

¹Department of Genetics, Harvard Medical School, Boston, Massachusetts.

Current Protocols in Human Genetics

|October 12, 2016

Summary

This summary is machine-generated.

Pronuclear Injection-based Targeted Transgenesis (PITT) offers a precise method for creating transgenic animal models. This technique avoids random DNA integration, ensuring safer and more reliable transgene expression compared to traditional methods.

Keywords:

Cre-LoxP recombination PITT PhiC31-attB/P integration pronuclear injection targeted transgenesis

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Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Published on: December 25, 2017

Area of Science:

Molecular Biology
Genetics
Animal Models

Background:

Traditional microinjection for transgenic models results in random DNA integration.
Random integration can cause gene disruption, variable copy numbers, and silenced transgenes.

Purpose of the Study:

To introduce Pronuclear Injection-based Targeted Transgenesis (PITT) as an improved method for generating transgenic animals.
To detail protocols and available tools for performing PITT.

Main Methods:

PITT targets transgenes to specific, safe genomic locations using enzymatic DNA transfer.
Utilizes systems like Cre-LoxP recombinase and PhiC31-attB/P integrase for DNA integration.

Main Results:

PITT overcomes the limitations of random integration associated with standard transgenesis.
Provides a reliable method for predictable transgene expression and genomic stability.

Conclusions:

PITT represents a significant advancement in generating transgenic animal models.
The described protocols and tools facilitate the adoption of PITT in research settings.