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Related Experiment Videos

Endothelial cell junctional integrity modulation by serotonin: an ultrastructural analysis.

R Mineau-Hanschke1, H B Hechtman, D Shepro

  • 1Boston University, Department of Biology, MA 02215.

Tissue & Cell
|January 1, 1989
PubMed
Summary

Serotonin (5-hydroxytryptamine, 5-HT) strengthens endothelial cell junctions by promoting actin filament assembly. This action enhances the integrity of the endothelial barrier, crucial for vascular health.

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Area of Science:

  • Vascular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Exogenous serotonin (5-hydroxytryptamine, 5-HT) is known to affect endothelial cell structural integrity.
  • The precise mechanisms by which 5-HT modulates endothelial cell-cell junctions require further elucidation.

Purpose of the Study:

  • To quantify the effect of 5-HT on bovine aortic endothelial cell (BAEC) junctional integrity.
  • To investigate the direct interaction of 5-HT with cellular components, specifically actin filaments.

Main Methods:

  • BAEC monolayers were treated with varying concentrations of 5-HT and histamine.
  • Intercellular openings were measured to assess junctional integrity.
  • Immunocytochemistry and electron microscopy were used to localize 5-HT binding to actin filaments.

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Main Results:

  • 5-HT significantly decreased intercellular openings in a dose-dependent manner, with maximal effect at 10(-6) M.
  • Histamine increased junctional openings, an effect reversed by 5-HT.
  • Electron microscopy showed 5-HT antibody binding to actin filaments, suggesting internalized 5-HT promotes actin network assembly.

Conclusions:

  • 5-HT plays a role in regulating the endothelial junctional barrier.
  • 5-HT promotes actin filament formation and stability, enhancing endothelial cell apposition.
  • These findings suggest 5-HT contributes to maintaining vascular integrity.