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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
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Efficient Management of High-Throughput Screening Libraries with SAVANAH.

Markus List^1,2, Marlene Pedersen Elnegaard¹, Steffen Schmidt¹

¹1 NanoCAN Center of Excellence, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.

SLAS Discovery : Advancing Life Sciences R & D

|October 13, 2016

Summary

High-throughput screening (HTS) generates numerous plates during serial dilution. SAVANAH is a new tool managing these molecular screening libraries, linking samples to experimental results for better analysis.

Keywords:

RNA interference RNAi cell-based assays database and data management phenotypic drug discovery sgRNA shRNA ultra-high-throughput screening

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Last Updated: Mar 13, 2026

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Area of Science:

Biomedical research
Pharmaceutical industry
Drug discovery

Background:

High-throughput screening (HTS) is crucial for drug discovery and biomedical research.
HTS relies on molecular libraries (siRNAs, small compounds) that require serial dilution.
Serial dilution of library plates leads to a significant increase in sample tracking complexity.

Purpose of the Study:

To introduce SAVANAH, the first software tool designed for managing molecular screening libraries during serial dilution.
To provide a solution for the challenge of tracking numerous plates and samples generated in HTS experiments.
To link sample information from library plates to experimental results from assay plates.

Main Methods:

Development of SAVANAH software for managing molecular screening libraries.
Implementation of features to link library sample information with assay plate experimental results.
Integration with R statistical environment and HiTSeekR for data analysis.

Main Results:

SAVANAH effectively manages molecular screening libraries across dilution series.
The tool facilitates the tracking of samples and plates generated during HTS.
Seamless export of results to R and HiTSeekR for downstream analysis is enabled.

Conclusions:

SAVANAH offers a comprehensive solution for managing and analyzing HTS experiments, particularly those involving serially diluted libraries.
The tool supports the HTS community by simplifying data management and analysis workflows.
SAVANAH enhances the efficiency of drug discovery and biomedical research by streamlining HTS processes.