Hydrogen peroxide in exhaled air: a source of error, a paradox and its resolution

The concentration of hydrogen peroxide (H₂O₂) in exhaled air has been reported to be elevated in asthma and chronic obstructive pulmonary disease (COPD), but results are inconsistent and difficult to reproduce. As H₂O₂ occurs in ambient air, we examined its association with exhaled H₂O₂ in human subjects. Exhaled breath condensate (EBC) of 12 COPD patients and nine healthy control subjects was collected either with an inhalation filter (efficiency 81%) or without. Ambient air condensate (AAC) was collected in parallel and samples were analysed for H₂O₂. Additionally, ambient H₂O₂ was recorded by an atmospheric measuring device (online fluorometric measurement). H₂O₂ concentration in AAC was significantly higher (p<0.001) than in EBC. AAC variations were concordant with the data from the atmospheric measuring instrument. In both subjects' groups, the inhalation filter reduced H₂O₂ values (p<0.01). Despite generally low levels in exhaled air, analysis by a mathematical model revealed a contribution from endogenous H₂O₂ production. The low H₂O₂ levels in exhaled air are explained by the reconditioning of H₂O₂-containing inhaled air in the airways. Inhaled H₂O₂ may be one factor in the heterogeneity and limited reproducibility of study results. A valid determination of endogenous H₂O₂ production requires inhalation filters.