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Protein Quantitation and Analysis of Purity
Eva M Campion1, Sinéad T Loughran2, Dermot Walls3,4
1Department of Life Sciences, Institute of Technology Sligo, Ash Lane, Sligo, Ireland. eva.campion@gmail.com.
Methods in Molecular Biology (Clifton, N.J.)
|October 13, 2016
Summary
Accurate protein quantitation and purity analysis are vital for research and clinical use. This study outlines common protein determination methods and introduces quantitative immuno-Polymerase Chain Reaction.
Area of Science:
- Biochemistry
- Molecular Biology
- Analytical Chemistry
Background:
- Accurate protein quantitation and purity assessment are fundamental in scientific research and clinical diagnostics.
- A wide array of techniques exists due to the diverse requirements for protein analysis.
Purpose of the Study:
- To provide an overview of commonly employed protein determination methodologies.
- To introduce the quantitative immuno-Polymerase Chain Reaction as a recently adapted technique for protein analysis.
Main Methods:
- Review and outline of established protein quantitation assays.
- Description of protocols for quantitative immuno-Polymerase Chain Reaction.
Main Results:
- Detailed protocols for standard protein measurement techniques are presented.
- The application and methodology of quantitative immuno-Polymerase Chain Reaction are explained.
Conclusions:
- The choice of protein assay depends on sensitivity, interfering substances, and protein characteristics.
- This work serves as a guide to selecting appropriate protein quantitation methods, including a novel immunoassay-PCR technique.
