Related Experiment Video
Updated: Mar 13, 2026
Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
Published on: May 26, 2023
Synthesis and Characterization of Sygyzium cumini Nanoparticles for Its Protective Potential in High Glucose-Induced
Neha Atale1, Sharad Saxena1, J Grace Nirmala2
1Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, Uttar Pradesh, 201307, India.
Abstract:
There exists a complex and multifactorial relationship between diabetes and cardiovascular disease. Hyperglycemia is an important factor imposing damage (glucose toxicity) on cardiac cell leading to diabetic cardiomyopathy. There are substantial clinical evidences on the adverse effects of conventional therapies in the prevention/treatment of diabetic cardiovascular complications. Currently, green-synthesized nanoparticles have emerged as a safe, efficient, and inexpensive alternative for therapeutic uses. The present study discloses the silver nanoparticle biosynthesizing capability and cardioprotective potential of Syzygium cumini seeds already reported to have antidiabetic properties. Newly generated silver nanoparticles S. cumini MSE silver nanoparticles (SmSNPs) were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), zeta sizer, X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. Using methanolic extract of S. cumini seeds, an average size of 40-100-nm nanoparticles with 43.02 nm and -19.6 mV zeta potential were synthesized. The crystalline nature of SmSNPs was identified by using XRD. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) assays revealed the antioxidative potential to be 66.87 (±0.7) % and 86.07 (±0.92) % compared to 60.29 (±0.02) % and 85.67 (±1.27) % for S. cumini MSE. In vitro study on glucose-stressed H9C2 cardiac cells showed restoration in cell size, nuclear morphology, and lipid peroxide formation upon treatment of SmSNPs. Our findings concluded that S. cumini MSE SmSNPs significantly suppress the glucose-induced cardiac stress in vitro by maintaining the cellular integrity and reducing the oxidative damages therefore establishing its therapeutic potential in diabetic cardiomyopathy.
Insights
Green-synthesized silver nanoparticles from Syzygium cumini seeds show promise in treating diabetic cardiomyopathy. These nanoparticles protect cardiac cells from glucose-induced stress and oxidative damage, offering a potential new therapy.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Cardiology
Background:
- Diabetes mellitus significantly increases cardiovascular disease risk.
- Hyperglycemia-induced damage (glucose toxicity) leads to diabetic cardiomyopathy.
- Conventional therapies have limitations in managing diabetic cardiovascular complications.
Purpose of the Study:
- To investigate the cardioprotective potential of silver nanoparticles biosynthesized using Syzygium cumini seeds.
- To characterize the synthesized silver nanoparticles (SmSNPs).
- To evaluate the efficacy of SmSNPs against in vitro glucose-induced cardiac stress.
Main Methods:
- Silver nanoparticles (SmSNPs) were synthesized using methanolic extract of Syzygium cumini seeds.
- Nanoparticles were characterized using UV-visible spectroscopy, SEM, zeta sizer, XRD, and FTIR.
- Antioxidant potential was assessed using DPPH and ABTS assays.
- In vitro study involved glucose-stressed H9C2 cardiac cells treated with SmSNPs.
Main Results:
- SmSNPs with an average size of 40-100 nm and a zeta potential of -19.6 mV were synthesized.
- XRD confirmed the crystalline nature of SmSNPs.
- DPPH and ABTS assays demonstrated significant antioxidant activity of SmSNPs.
- SmSNPs treatment restored cell size, nuclear morphology, and reduced lipid peroxide formation in glucose-stressed cardiac cells.
Conclusions:
- Syzygium cumini seed-derived silver nanoparticles (SmSNPs) exhibit significant cardioprotective effects.
- SmSNPs effectively suppress glucose-induced cardiac stress by maintaining cellular integrity and reducing oxidative damage.
- These findings highlight the therapeutic potential of SmSNPs for managing diabetic cardiomyopathy.
More Related Videos
06:34New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells
Published on: October 9, 2017
05:58Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model
Published on: April 18, 2025
