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Artificial oocyte activation after intracytoplasmic morphologically selected sperm injection: A prospective
Batu Aydinuraz1, Enver Kerem Dirican2, Safak Olgan2
1a IVF Unit, Private Gelecek Center for Assisted Reproductive Technologies , Antalya , Turkey.
Human Fertility (Cambridge, England)
|October 14, 2016
Summary
Artificial oocyte activation (AOA) after intracytoplasmic morphologically selected sperm injection (IMSI) did not improve fertilization rates. In fact, AOA may decrease the development of top-quality embryos, suggesting its use should be limited to specific cases.
Area of Science:
- Reproductive Medicine
- Embryology
- In Vitro Fertilization
Background:
- Intracytoplasmic morphologically selected sperm injection (IMSI) is used in assisted reproductive technologies.
- Artificial oocyte activation (AOA) aims to improve fertilization rates in cases of previous failure.
- The combined effect of IMSI and AOA on embryo development requires further investigation.
Purpose of the Study:
- To evaluate the impact of AOA following IMSI on fertilization, cleavage rates, and embryo quality.
- To determine if AOA enhances outcomes in patients with a history of low fertilization rates and teratozoospermia.
Main Methods:
- A prospective study involving 194 oocytes from 21 IVF cycles.
- Oocytes were randomly assigned to either IMSI with AOA (study group) or IMSI without AOA (control group).
- Fertilization, cleavage rates, and embryo quality (top quality embryo development) were compared between groups.
Main Results:
- No significant difference in fertilization or cleavage rates between the AOA and control groups.
- Fertilized oocytes in the AOA group showed a significantly lower likelihood of developing into top-quality embryos.
- Cycle-based analysis also revealed a lower rate of top-quality embryo development in the AOA group.
Conclusions:
- AOA following IMSI does not appear to improve fertilization rates.
- AOA may negatively impact the potential for a fertilized oocyte to develop into a top-quality embryo.
- AOA should be reserved for patients with diagnosed oocyte activation deficiencies.
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