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Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
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Liquid Handling Optimization in High-Throughput Biodosimetry Tool.

Dakai Bian1, Jason C Tsui2, Mikhail Repin3

  • 1Department of Mechanical Engineering, Columbia University, New York, NY 10027

Journal of Medical Devices
|October 18, 2016
PubMed
Summary
This summary is machine-generated.

A robotically based automated biodosimetry tool (RABiT) was developed for rapid radiation dose assessment. Optimized liquid handling in RABiT ensures accurate micronucleus assay results for biodosimetry.

Keywords:
automated biodosimetry toolmicronucleus assayplatform-dependent liquid handling

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Area of Science:

  • Biotechnology
  • Radiation Biology
  • Automation

Background:

  • High-speed biodosimetric assays are crucial for triage and therapy after radiological events.
  • Existing methods face challenges in automation and liquid handling precision.

Purpose of the Study:

  • To develop and validate a robotically based automated biodosimetry tool (RABiT).
  • To address liquid handling challenges in adapting the micronucleus assay to V-shaped plates for automated processing.

Main Methods:

  • Experimental and numerical investigations of liquid handling phenomena (splashing, dispensing, aspiration).
  • Incorporation of surface tension and contact angle in discrete and multiphase numerical models.
  • Optimization of experimental parameters (nozzle and aspiration speeds) based on numerical findings.

Main Results:

  • Identified and mitigated issues of cell splashing, poor distribution, and cell loss during automated liquid handling.
  • Optimized parameters demonstrated a clear relationship between nozzle/aspiration speeds and liquid handling outcomes.
  • Micronucleus assay results in RABiT-prepared samples showed dose-dependent accuracy comparable to manual methods.

Conclusions:

  • The robotically based automated biodosimetry tool (RABiT) successfully adapted the micronucleus assay for automated processing.
  • Optimized liquid handling protocols ensure reliable and efficient biodosimetric analysis.
  • RABiT provides a viable solution for high-throughput biodosimetry in radiological emergencies.