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Experimental paratuberculosis (Johne's disease)--studies on biochemical parameters in cattle
Archiv Fur Experimentelle Veterinarmedizin
|May 1, 1989
Summary
This study shows that reduced total protein, albumin, triglycerides, and cholesterol, along with elevated muscle and liver enzymes, indicate advanced paratuberculosis in calves. These markers can aid in diagnosing the disease.
Area of Science:
- Veterinary Medicine
- Animal Pathology
- Biochemistry
Background:
- Paratuberculosis, caused by Mycobacterium paratuberculosis, is a chronic intestinal infection in ruminants.
- Diagnosis in advanced stages can be challenging, necessitating biochemical markers.
Purpose of the Study:
- To investigate biochemical and enzymatic changes in calves with paratuberculosis.
- To identify potential diagnostic markers for advanced-stage paratuberculosis.
Main Methods:
- Ten male Holstein-Friesian calves with natural Mycobacterium paratuberculosis infection were experimentally re-infected.
- Monthly blood analyses were performed from day 160 to 400 post-infection.
- Measurements included total protein, albumin, cholesterol, triglycerides, Zn, Cu, and various enzyme activities (LDH, AST, ALT, CK, ALD, etc.).
Main Results:
- By day 400, total protein, albumin, triglycerides, and cholesterol levels decreased, correlating with digestive and absorption issues.
- Increased activities of creatine kinase (CK), aldolase (ALD), lactate dehydrogenase (LDH), and aminotransferases (AST, ALT) indicated skeletal muscle and/or liver damage.
- Serum CK and ALD activities, along with triglyceride and total protein concentrations, showed potential as diagnostic aids.
Conclusions:
- Biochemical profiles, particularly decreased total protein and triglycerides, and increased CK and ALD, are indicative of advanced paratuberculosis.
- These specific markers may assist in the diagnosis of paratuberculosis in its later stages.