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Updated: Mar 13, 2026

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
Published on: April 17, 2016
Increased callose deposition in plants lacking DYNAMIN-RELATED PROTEIN 2B is dependent upon POWDERY MILDEW RESISTANT
Michelle E Leslie1, Sean W Rogers1, Antje Heese1
1a Division of Biochemistry, Interdisciplinary Plant Group, University of Missouri-Columbia , Columbia , MO , USA.
Abstract:
Callose deposition within the cell wall is a well-documented plant immune response to pathogenic organisms as well as to pathogen-/microbe- associated molecular patterns (P/MAMPs). However, the molecular mechanisms that modulate pathogen-induced callose deposition are less understood. We reported previously that Arabidopsis plants lacking the vesicle trafficking component DYNAMIN-RELATED PROTEIN 2B (DRP2B) display increased callose deposition in response to the PAMP flg22. Here, we show that increased number of flg22-induced callose deposits in drp2b leaves is fully dependent on the callose synthase POWDERY MILDEW RESISTANT 4 (PMR4). We propose that in addition to functioning in flg22-induced endocytosis of the plant receptor, FLAGELLIN SENSING 2, DRP2B may regulate the trafficking of proteins involved in callose synthesis, such as PMR4, and/or callose degradation.
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