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Bromodeoxyuridine amplifies free-radical-mediated DNA damage
M Poot1, P S Rabinovitch, H Hoehn
1Department of Human Genetics, University of Würzburg, Federal Republic of Germany.
The Biochemical Journal
|July 1, 1989
Summary
Elevated oxygen and paraquat cause cell cycle arrest, enhanced by bromodeoxyuridine (BrdU). This BrdU-dependency offers a sensitive assay for detecting oxygen free radical-induced DNA damage.
Area of Science:
- Cell Biology
- Toxicology
- Molecular Biology
Background:
- Elevated oxygen and paraquat induce cell cycle arrest at the G2 phase.
- Bromodeoxyuridine (BrdU) enhances this G2 phase arrest.
- Oxygen free radicals are implicated in cellular damage.
Purpose of the Study:
- To investigate the mechanism of BrdU-enhanced G2 arrest.
- To determine if lipid peroxidation mediates the BrdU-dependent G2 arrest.
- To establish a cell-based assay for detecting oxygen free radical-induced DNA damage.
Main Methods:
- Cell culture experiments using elevated oxygen and paraquat.
- Treatment with bromodeoxyuridine (BrdU).
- Experiments with cumene hydroperoxide and vitamin E to assess lipid peroxidation and free radical scavenging.
Main Results:
- Bromodeoxyuridine (BrdU) significantly enhances G2 phase cell cycle arrest induced by oxygen and paraquat.
- The BrdU-dependent G2 arrest is independent of lipid peroxidation.
- Cumene hydroperoxide and vitamin E experiments confirmed the non-involvement of lipid peroxidation.
Conclusions:
- The BrdU-dependent G2 arrest is a specific response to oxygen free radical-induced DNA damage.
- This phenomenon provides a sensitive cell biological assay for detecting DNA damage caused by oxygen free radicals.
- The findings contribute to understanding cellular responses to oxidative stress.