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Bovine annulus fibrosus cell lines isolated from intervertebral discs
1Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699, USA.
Genomics Data
|October 19, 2016
Summary
Researchers isolated bovine annulus fibrosus cells without enzymatic digestion. These cells, viable past passage 10 and after freeze-thawing, maintain native gene expression, offering a promising cell source for tissue engineering.
Area of Science:
- Veterinary Medicine
- Cell Biology
- Biomaterials Science
Background:
- The bovine intervertebral disc consists of annulus fibrosus (AF) and nucleus pulposus (NP).
- Current cell isolation methods often involve enzymatic digestion, potentially altering cell properties.
Purpose of the Study:
- To isolate and characterize primary bovine AF cells without enzymatic digestion.
- To assess the proliferative capacity, viability, and gene expression of these cells.
Main Methods:
- Isolation of passage 0 (P0) cells directly from bovine AF tissue.
- Culture in DMEM + 10% FCS on gelatin-coated/uncoated plates.
- Assessment of cell senescence and freeze-thaw survival.
- Preliminary gene expression analysis (Col1a1, Col2a1) using PISH.
Main Results:
- Successful isolation of AF cells without enzymatic digestion.
- Cells proliferated beyond P10 without senescence.
- Cells survived freeze-thawing procedures.
- PISH confirmed in vivo-like expression of Col1a1 and Col2a1.
Conclusions:
- A novel, non-enzymatic method yields robust bovine AF cells.
- These cells demonstrate high viability, proliferative potential, and stable gene expression.
- This cell source is suitable for intervertebral disc tissue engineering and research.
