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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Corepressor diversification by alternative mRNA splicing is species specific.

Martin L Privalsky1, Chelsea A Snyder2, Michael L Goodson2

  • 1Department of Microbiology and Molecular Genetics, College of Biological Sciences, University of California at Davis, One Shields Avenue, Davis, CA, 95616, USA. mlprivalsky@ucdavis.edu.

BMC Evolutionary Biology
|October 21, 2016
PubMed
Summary

Alternative splicing diversifies corepressor functions. SMRT alternative splicing is recent evolutionarily, while NCoR

Keywords:
CorepressorEvolutionNCoRSMRTmRNA splicing

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Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Genetics

Background:

  • SMRT (silencing mediator of retinoic acid receptor) and NCoR (nuclear receptor corepressor 1) are key transcriptional corepressors.
  • These corepressors mediate gene silencing by various transcription factors, including nuclear hormone receptors.
  • Alternative mRNA splicing in mice generates diverse SMRT and NCoR protein variants with distinct tissue-specific functions.

Purpose of the Study:

  • To investigate the evolutionary history of alternative splicing in SMRT and NCoR.
  • To understand how alternative splicing contributes to the functional diversification of these corepressors across species.

Main Methods:

  • Comparative analysis of SMRT and NCoR alternative splicing patterns across different vertebrate species.
  • Identification of conserved and novel alternative splicing sites.

Main Results:

  • SMRT alternative splicing is a relatively recent evolutionary event, with limited utilization in fish, birds, reptiles, and marsupials, but extensive use in placental mammals.
  • NCoR alternative splicing, observed in mice and linked to metabolic regulation, appears to have evolved independently and later than SMRT, demonstrating convergent evolution.
  • The extent of alternative splicing for both SMRT and NCoR varies significantly across the studied taxa.

Conclusions:

  • Alternative splicing has played a crucial role in diversifying the functions of SMRT and NCoR throughout evolution.
  • This diversification allows for specialized roles of these corepressors in different tissues and species.
  • The independent evolution of NCoR alternative splicing highlights convergent evolutionary strategies for functional adaptation.