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All-optical optoacoustic microscopy based on probe beam deflection technique.

Saher M Maswadi1, Bennett L Ibey2, Caleb C Roth3

  • 1Oak Ridge Institute for Science and Education, 4141 Petroleum Road, JBSA Fort Sam Houston, TX 78234, USA; Department of Physics and Astronomy, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA; EchoLase, Inc., 5234 Tomas Circle, San Antonio, TX 78240, USA.

Photoacoustics
|October 21, 2016
PubMed
Summary

Optoacoustic microscopy utilizing probe beam deflection technique (PBDT) offers an all-optical alternative to traditional transducers. This advanced method enhances imaging sensitivity and resolution for biological samples.

Keywords:
All optical optoacoustic systemBackward mode optoacoustic microscopyNon-contact acoustic sensorOptical resolution photoacoustic imagingOptoacoustic tomographyProbe beam deflection technique

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Area of Science:

  • Biomedical Optics
  • Acoustic Imaging
  • Microscopy

Background:

  • Conventional optoacoustic microscopy often relies on piezoelectric transducers.
  • These transducers can have limitations in signal coupling and bandwidth.
  • Exploring alternative detection methods is crucial for advancing OA microscopy.

Purpose of the Study:

  • To investigate the probe beam deflection technique (PBDT) as an all-optical detection method for optoacoustic microscopy.
  • To evaluate the advantages of PBDT over conventional piezoelectric transducers for OA imaging.
  • To demonstrate the capability of PBDT-based OA microscopy in resolving fine biological structures.

Main Methods:

  • An all-optical optoacoustic microscopy system was developed using the probe beam deflection technique (PBDT).
  • Laser-induced acoustic signals were detected via optical probing.
  • The system's performance was characterized by its sensitivity and noise equivalent pressure (NEP).
  • Histological sections of cardiac muscle were imaged to assess resolution.

Main Results:

  • PBDT demonstrated efficient coupling of laser energy and undistorted acoustic wave detection.
  • The system achieved diffraction-limited lateral resolution due to an unimpeded optical path.
  • The PBDT sensor exhibited a sensitivity of 22 μV/Pa and an NEP of 11.4 Pa, comparable to other advanced detectors.
  • Micron-size details in cardiac muscle tissue were successfully resolved.

Conclusions:

  • The probe beam deflection technique (PBDT) presents a viable and advantageous all-optical alternative for optoacoustic microscopy.
  • PBDT offers high sensitivity, ultrawide bandwidth, and excellent resolution, overcoming limitations of piezoelectric transducers.
  • This technique holds significant potential for high-resolution imaging of biological tissues.