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Updated: Mar 13, 2026
Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Published on: July 11, 2012
Design of a multi-enzyme reaction on an electrode surface for an L-glutamate biofuel anode
Hiroaki Sakamoto1, Tomohiro Komatsu2, Koji Yamasaki2
1Tenure-Track Program for Innovation Research, University of Fukui, Fukui, Japan.
Objectives:
To design and construct a novel bio-anode electrode based on the oxidation of glutamic acid to produce 2-oxoglutarate, generating two electrons from NADH.
Results:
Efficient enzyme reaction and electron transfer were observed owing to immobilization of the two enzymes using a mixed self-assembled monolayer. The ratio of the immobilized enzymes was an important factor affecting the efficiency of the system; thus, we quantified the amounts of immobilized enzyme using a quartz crystal microbalance to further evaluate the electrochemical reaction. The electrochemical reaction proceeded efficiently when approximately equimolar amounts of the enzyme were on the electrode. The largest oxidation peak current increase (171 nA) was observed under these conditions.
Conclusion:
Efficient multi-enzyme reaction on the electrode surface has been achieved which is applicable for biofuel cell application.
