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Parallel Control over Surface Charge and Wettability Using Polyelectrolyte Architecture: Effect on Protein Adsorption
Shanshan Guo1, Xiaoying Zhu2,3, Min Li4
1NUS Graduate School for Integrative Science and Engineering, National University of Singapore , Kent Ridge, Singapore 117576.
ACS Applied Materials & Interfaces
|November 1, 2016
Summary
Surface charge and wettability independently influence protein adsorption and cell adhesion. Combined, positive charge and hydrophilicity maximize fibroblast adhesion, while negative charge and hydrophobicity minimize it.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Cell Biology
Background:
- Surface charge and wettability are key factors in protein adsorption and cell adhesion.
- Previous studies lack systematic, quantitative comparisons of their independent and cooperative effects.
Purpose of the Study:
- To investigate the independent and combined effects of surface charge and wettability on protein adsorption and cell adhesion.
- To develop a method for two-dimensional control over surface properties.
Main Methods:
- Utilized layer-by-layer (LbL) assembly to control polyion charge density and side-chain chemistry.
- Created a 2D property matrix with controlled isoelectric points (5-9) and water contact angles (35-70°).
- Eliminated interferential factors like surface roughness.
Main Results:
- Protein adsorption shows thresholds for wettability and electrostatic forces; beyond these, individual effects dominate.
- Fibroblast cell adhesion is influenced by both charge and wettability.
- Maximal cell adhesion observed with positive charge and hydrophilicity; minimal with negative charge and hydrophobicity.
Conclusions:
- Surface charge and wettability play distinct and interactive roles in interfacial phenomena.
- The developed 2D control strategy provides a reference for studying protein adsorption and cell adhesion.
- Findings offer insights for designing biomaterials with tailored surface properties.
