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Published on: September 26, 2013
Altered expression of interleukin-17A and its targeting microRNAs in oral lichen planus: a pilot study
Zhengyu Shen1, Chenping Zhang2, Zengtong Zhou3
1Department of Dermatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Objective:
To investigate the potential role of interleukin (IL)-17A and its targeting microRNAs (miRNAs) in oral lichen planus (OLP).
Study Design:
We compared the IL-17A expression levels in biopsies from patients with OLP (n = 50) against normal controls (n = 19) by using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot analyses, identified the targeting miRNAs of IL-17A, and examined the miRNA expression levels in OLP.
Results:
IL-17A expression was significantly increased in patients with OLP compared with controls. The dual-luciferase reporter assay system demonstrated that miR-635 and miR-578 were the target miRNAs of IL-17A in human embryonic kidney 293 cells, which was consistent with predictions from bioinformatics software analyses. In contrast with IL-17A, the expression levels of miR-635 and miR-578 in OLP were significantly decreased compared with controls.
Conclusions:
This pilot study found that low levels of miR-635 and miR-578 expression were associated with high expression of IL-17A, suggesting that IL-17A and its targeting miRNAs contribute to the pathogenesis of OLP.
Insights
This study found that interleukin-17A (IL-17A) is upregulated in oral lichen planus (OLP). Decreased levels of targeting microRNAs (miRNAs), miR-635 and miR-578, correlate with increased IL-17A in OLP pathogenesis.
Area of Science:
- Immunology
- Molecular Biology
- Oral Pathology
Background:
- Oral lichen planus (OLP) is a chronic inflammatory condition.
- The role of interleukin-17A (IL-17A) and its regulatory microRNAs (miRNAs) in OLP pathogenesis requires further elucidation.
Purpose of the Study:
- To investigate the expression of IL-17A and its targeting miRNAs in OLP.
- To determine the relationship between IL-17A and specific miRNAs in OLP.
Main Methods:
- Compared IL-17A expression in OLP biopsies (n=50) versus controls (n=19) using RT-qPCR and Western blot.
- Identified IL-17A targeting miRNAs (miR-635, miR-578) via dual-luciferase reporter assay and bioinformatics.
- Examined miR-635 and miR-578 expression levels in OLP tissues.
Main Results:
- IL-17A expression was significantly elevated in OLP patients compared to controls.
- miR-635 and miR-578 were confirmed as direct targets of IL-17A.
- Expression of miR-635 and miR-578 was significantly reduced in OLP tissues.
Conclusions:
- Low expression of miR-635 and miR-578 is associated with high IL-17A expression in OLP.
- IL-17A and its targeting miRNAs are implicated in the pathogenesis of oral lichen planus.
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