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Related Experiment Videos

Flow cytometry and phytoplankton.

D A Phinney1, T L Cucci

  • 1J.J. MacIsaac Flow Cytometry/Cell Sorting Facility, Bigelow Laboratory for Ocean Sciences, McKown Point, West Boothbay Harbor, Maine 04575.

Cytometry
|September 1, 1989
PubMed
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Flow cytometry and sorting are crucial for aquatic research, enabling detailed analysis of individual particles. Using standardized reference materials ensures reliable and comparable data on aquatic particle distributions.

Area of Science:

  • Environmental Science
  • Marine Biology
  • Biotechnology

Background:

  • Flow cytometry and sorting are increasingly vital tools in aquatic research.
  • Simultaneous measurement of cell size, fluorescence, and light scatter properties offers direct application to current aquatic research topics.
  • Flow sorting enables isolation of specific cell subsets for further conventional analysis.

Purpose of the Study:

  • To discuss the complexities and analytical constraints of applying flow cytometry to aquatic samples.
  • To highlight the importance of reference materials for validating flow cytometric analyses.
  • To explore the use of standards and controls in allometric and ataxonomic methods for aquatic particle characterization.

Main Methods:

  • Utilizing flow cytometry for simultaneous measurements of algal cell size and pigment autofluorescence.

Related Experiment Videos

  • Employing instrument standards, fluorescence controls, and internal stain standards for data validation.
  • Applying allometric (cell size vs. pigment fluorescence) and ataxonomic (pigment color groups) methods.

    • Main Results:

    • Flow cytometric measurements of algal cell size and pigment autofluorescence are relative and instrument-dependent.
    • Reference materials are essential for establishing the validity of flow cytometric analyses.
    • The selection and application of standards and controls are critical for accurate interpretation.

    Conclusions:

    • Widespread acceptance of specific reference materials will lead to comparable datasets for aquatic particle distributions.
    • Standardization in flow cytometry is key to advancing aquatic research.
    • Consistent application of validated methods ensures reliable ecological data.